{"id":177,"date":"2018-07-19T22:44:34","date_gmt":"2018-07-19T19:44:34","guid":{"rendered":"http:\/\/2019.brs.org.tr\/?page_id=177"},"modified":"2018-07-21T21:28:30","modified_gmt":"2018-07-21T18:28:30","slug":"patch-clamp","status":"publish","type":"page","link":"https:\/\/2023.brs.org.tr\/index.php\/patch-clamp\/","title":{"rendered":"Patch Clamp"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-216\" src=\"http:\/\/2019.brs.org.tr\/wp-content\/uploads\/2018\/07\/patch-2.jpg\" alt=\"\" width=\"2000\" height=\"1500\" srcset=\"https:\/\/2023.brs.org.tr\/wp-content\/uploads\/2018\/07\/patch-2.jpg 2000w, https:\/\/2023.brs.org.tr\/wp-content\/uploads\/2018\/07\/patch-2-300x225.jpg 300w, https:\/\/2023.brs.org.tr\/wp-content\/uploads\/2018\/07\/patch-2-768x576.jpg 768w, https:\/\/2023.brs.org.tr\/wp-content\/uploads\/2018\/07\/patch-2-1024x768.jpg 1024w, https:\/\/2023.brs.org.tr\/wp-content\/uploads\/2018\/07\/patch-2-345x259.jpg 345w\" sizes=\"auto, (max-width: 2000px) 100vw, 2000px\" \/><\/p>\n<p>Patch clamp tekni\u011fi; h\u00fccre zar\u0131 potansiyeli belli bir de\u011fere kenetlenmi\u015f durumdayken iyonlar\u0131n, h\u00fccre zar\u0131 \u00fczerinde bulunan iyon kanallar\u0131ndan ge\u00e7erken meydana getirdi\u011fi elektrik ak\u0131m\u0131n\u0131n \u00f6l\u00e7\u00fclmesi prensibine dayanan elektrfizyolojik bir y\u00f6ntemdir. Bu y\u00f6ntem, voltaj-kenetleme tekni\u011finin daha da geli\u015ftirilmi\u015f hali olmakla beraber \u00f6zellikle n\u00f6ronlar, kardiyomiyositler kas h\u00fccreleri ve pankreatik beta h\u00fccreleri gibi uyar\u0131labilir tipteki h\u00fccreler \u00fczerinde iyon kanal\u0131 agonist ve antagonistleri \u00fczerine ara\u015ft\u0131rma yapmak i\u00e7in en uygun en g\u00fcncel y\u00f6ntemlerden biridir. Patch-clamp y\u00f6ntemi ilk kez\u00a0Erwin Neher and Bert Sakmann isimli iki alman bilim insan\u0131 taraf\u0131ndan geli\u015ftirilmi\u015f olup bu bulu\u015flar\u0131yla 1991 y\u0131l\u0131nda Fizyoloji ve T\u0131p Alan\u0131nda Nobel \u00f6d\u00fcl\u00fcne lay\u0131k g\u00f6r\u00fclm\u00fc\u015flerdir.<\/p>\n<h3><strong>Patch Clamp Kurs\u00a0Program\u0131<\/strong><\/h3>\n<p>1. Ders Patch Clamp Nedir ? Cihazlar\u0131n tan\u0131t\u0131m\u0131 Patch Clamp\u2019te \u00e7al\u0131\u015f\u0131lacak kanala uygun \u00f6zelliklerin anlat\u0131m\u0131 (Agonist, antagonist ve dozlar\u0131)<br \/>\n2. Ders Patch Clamp \u00e7al\u0131\u015fmalar\u0131nda kullan\u0131lan intracellular ve extracellular sol\u00fcsyonlar\u0131n haz\u0131rlanmas\u0131.Kanala uygun agonist ve antagonistlerin haz\u0131rlanmas\u0131<br \/>\n3. Ders Borosilikat t\u00fcplerden Patch pipeti yap\u0131lmas\u0131, pipete uygun ortam s\u0131v\u0131s\u0131n\u0131n ilave edilmesi Primer K\u00fclt\u00fcrlerde Patch Clamp kay\u0131tlar\u0131n\u0131n al\u0131nmas\u0131<br \/>\n4. Ders DRG h\u00fccrelerinde Patch Clamp kay\u0131tlar\u0131n\u0131n al\u0131nmas\u0131<br \/>\n5. Ders DRG h\u00fccrelerinde Patch Clamp kay\u0131tlar\u0131n\u0131n al\u0131nmas\u0131<br \/>\n6. Ders DRG h\u00fccrelerinde Patch Clamp kay\u0131tlar\u0131n\u0131n al\u0131nmas\u0131<br \/>\n7. Ders Sekonder k\u00fclt\u00fcrlerde Patch Clamp kay\u0131tlar\u0131 i\u00e7in h\u00fccre hatlar\u0131n\u0131n haz\u0131rlanmas\u0131 ve kayda ba\u015flama<br \/>\n8. Ders N\u00f6ronal h\u00fccre hatlar\u0131nda Patch Clamp kay\u0131tlar\u0131n\u0131n al\u0131nmas\u0131<br \/>\n9. Ders Al\u0131nan kay\u0131tlar\u0131n analizi ve yay\u0131na uygun formata d\u00f6n\u00fc\u015ft\u00fcr\u00fclmesi<br \/>\n10. Ders Tart\u0131\u015fma ve Tavsiyeler<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Patch clamp tekni\u011fi; h\u00fccre zar\u0131 potansiyeli belli bir de\u011fere kenetlenmi\u015f durumdayken iyonlar\u0131n, h\u00fccre zar\u0131 \u00fczerinde bulunan iyon kanallar\u0131ndan ge\u00e7erken meydana getirdi\u011fi elektrik ak\u0131m\u0131n\u0131n \u00f6l\u00e7\u00fclmesi prensibine dayanan elektrfizyolojik bir y\u00f6ntemdir. Bu y\u00f6ntem, voltaj-kenetleme tekni\u011finin daha da geli\u015ftirilmi\u015f hali olmakla beraber \u00f6zellikle n\u00f6ronlar, kardiyomiyositler kas h\u00fccreleri ve pankreatik beta h\u00fccreleri gibi uyar\u0131labilir tipteki h\u00fccreler \u00fczerinde iyon kanal\u0131 agonist ve antagonistleri \u00fczerine ara\u015ft\u0131rma yapmak i\u00e7in en uygun en g\u00fcncel y\u00f6ntemlerden biridir. Patch-clamp y\u00f6ntemi&hellip; <\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-177","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/2023.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/177","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/2023.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/2023.brs.org.tr\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/2023.brs.org.tr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/2023.brs.org.tr\/index.php\/wp-json\/wp\/v2\/comments?post=177"}],"version-history":[{"count":4,"href":"https:\/\/2023.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/177\/revisions"}],"predecessor-version":[{"id":358,"href":"https:\/\/2023.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/177\/revisions\/358"}],"wp:attachment":[{"href":"https:\/\/2023.brs.org.tr\/index.php\/wp-json\/wp\/v2\/media?parent=177"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}