The understanding of cellular functional metabolic processes is important to resolve pathological machinery of the disease. The aim of the cell culture studies to produce the similar cell types to functionally specific tissues and provide as many cells as possible to experimental utilization in the various lab researches under in vitro conditions. Hence, cellular specify to any tissue, organ, system or mimicking any pathology, investigators can make inferences to physiological functions by using cell culture. Although it provides more limited information and data on the studied case, it is more optimized than in vivo studies because the results obtained in cell culture are less than other physiological variables. This advantage has therefore been recognized as the first step in the transition to in vivo and phase studies from cell culture studies. In addition, the researcher also has the advantage in cell culture studies of more free thinking and working because of the high cost of phase studies, difficulty in application, the need to reach a sufficient number of statistically samples, and ethical concerns.
Cell Culture Course Program
Lecture 1 Introduction to Cell Culture, Short Explanation about Cell Lines and Theoretical Process
Lecture 2 Cell Culture Lab Materials, Functions, Working Safety, Continuing the Sterile Conditions
Lecture 3 Growth Media Contents for Different Cell Lines and Medium Preparation
Lecture 4 Thawing the Cells from Cryo Vials, Seeding to the Culture Flasks, Starting to Incubation
Lecture 5 Observations of the Cells, Changing the Growth Medium, Continuing to Incubation. Removing of Previously Seeded and Fully Confluence Cells and Subculturing
Lecture 6 Observations of the Cells, Changing the Growth Medium, Continuing to Incubation. Removing of Previously Seeded and Fully Confluence Cells and Subculturing
Lecture 7 Observations of the Cells, Changing the Growth Medium, Continuing to Incubation. Removing of Previously Seeded and Fully Confluence Cells and Subculturing
Lecture 8 Preparation of Freezing Solution and Freezing of the Cells
Lecture 9 Research Techniques in Cell Culture (Transfection and Cell Viability Assays)
Lecture 10 Discussion and Recommendations