Western Blot Course Program  

Lecture 1 İntroduction to Western Blotting

·        Principles

·        Data Analysis

·        Troubleshooting

Lecture 2 Introduction to WB equipment
Lecture 3 Sample and Gel preperation
Lecture 4 Loading samples and running the gel (Electrophoresis)
Lecture 5 Electro Transfer (Transfer of proteins to membrane)
Lecture 6 Blocking the membrane and Incubation with the primary antibody
Lecture 7 Incubation with Secondary Antibody
Lecture 8 Imaging ( Dark room applications ve Imaging System app.)
Lecture 9 Interpretation of results
Lecture 10 Group discussions and advice